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The Preclinical Imaging core provides state-of-the-art in vivo imaging technology and infrastructure to support basic and preclinical research. We offer a diverse array of imaging modalities, including ultrasound and optical imaging, MRI, micro-CT and multiphoton microscopy.

Training Opportunities

We operate a diverse array of imaging modalities as a shared resource at Fred Hutch. As part of the Translational Bioimaging Core, or TBIC, a Fred Hutch/University of Washington Cancer Consortium agreement with UW, we collaborate closely with other research centers in the Seattle area to support investigators performing basic and preclinical research. Our imaging modalities capture physiological and/or anatomical data with a wide range of spatial and temporal resolutions and imaging depths, ensuring we can support any basic or preclinical study.

We offer training for researchers to operate most equipment independently. We also image for researchers and offer consultation services in data analysis, research design and troubleshooting. Please contact us at to learn more. 

Learning Modules

Virtual learning modules available on Hutch Learning (requires Fred Hutch credentials to access).

Grants & Publications

Please ensure that you credit the use of proper grants and services in your manuscripts. Fred Hutch Shared Resources may not be allowed to credit your paper in our next Cancer Center Support Grant (CCSG) renewal if this is not done. This is important and your responsibility!

Recommended language for acknowledgement of:

For any use of Preclinical Imaging services or instruments:

This research was supported by the Preclinical Imaging Shared Resource of the Fred Hutch/University of Washington Cancer Consortium (P30 CA015704).

For use of 7T MRI services: 

This research was supported by the Preclinical Imaging Shared Resource of the Fred Hutch/University of Washington Cancer Consortium (P30 CA015704) and (3T/7T MRI SIG: NIH S10OD26919)

For detailed CCSG grant information, please visit the CCSG website or contact the Cancer Consortium Administration.

Note: For more information on NIH compliance, please contact the Arnold Library staff for NIH Public Access compliance assistance to researchers and Pls.  They can be reached at:  / 206.667.4314 

Downloadable Content


2021 2PM Training Guide
Training guide that outlines basic operations with the two-photon microscope (2PM aka the Multiphoton Microscope, MPM) in BE-154 within Comparative Medicine's animal vivarium.

* 2021 2PM Training Guide

Hutch Learning

* Multiphoton Microscopy Hutch Learning Course Printout
* IVIS Hutch Learning Course Printout
* Echo MRI Hutch Learning Course Printout
* Vevo 2100 Ultrasound Hutch Learning Course Printout


IVIS Training Guide
Training Guide for IVIS Usage

BSL2 IVIS Procedures
Procedure guidelines for the BSL2 IVIS.

IVIS Request Form
Request form for IVIS Assisted Imaging

2009 Inoue IP versus SQ Luciferin Injection Comparison
Publication - IP vs. SQ Luciferin Injections

IVIS Spectrum Presentation
Introductory Presentation for IVIS Spectrum and Living Image Software

Luciferin Curve
Guidelines to help you determine the Luciferin Kinetic Curve for your model.

Luciferin SQ Route due to IP Injection Failure
Publication - Subcutaneous administration of D-Luciferin is an effective alternative to intraperitoneal injection in bioluminescence imaging of xenograft tumors in nude mice.

SOP Luciferin Prep of In Vitro and In Vivo Bioluminescent Assays
Standard Operationg Procedure for preparing Luciferin for In Vitro and In Vivo Bioluminescent Assays


HU Calibrations After a Scan
Guidelines for HU calibrations after a MicroCT scan

Analyze 14 Introduction
Introductory presentation for the Analyze 14 software

A14 Lung Segmentation Workflow
Guidelines for Lung Segmentation on Analyze 14

Living Image Data Analysis Guide
IVIS-CT coregistration data analysis guide with Living Image (only available on Steam Plant IVIS PC)

microCT Request Form
Scheduling form for imaging on the microCT

QGX2 Software Navigation
Outline of basic navigation for QGX2 software

Steam Plant Transfer Request Form
Note: If you are transferring your mice to and from the Steam Plant for CT imaging (i.e. they are housed in Weintraub and they will be returned back to Weintraub after CT imaging is completed), please fill out this form twice. Once with details to transfer to the Steam Plant and a second time to transfer back to Weintraub.


* 7T MRI Request Form

7T/3T MRI Request Form
Request form for 7T/3T MRI usage

MRI Image Denoising Algorithm


* Ultrasound Export Data Instructions

Ultrasound Export Data Instructions
Instructions for Ultrasound Data Export
Note: Comparative Medicine is not responsible for any unsaved or lost data on any imaging equipment


Analysis Software Locations and Availability
Analysis Software availbility by location.

Internal Modalitie At FHSRCM
Preclinical Imaging Modalities Available in Comparative Medicine at Fred Hutch