All employees handling hazardous materials must wear the appropriate PPE when necessary. Standard lab PPE includes a lab coat, safety glasses, and gloves such as powder-free SafeSkin, N-dex, or NeoPro, and closed shoes. Due to possible chemical exposure to legs, all workers handling hazardous materials should wear pants or the equivalent.
The two most common routes of exposure when handling chemicals in the lab are inhalation and skin contact or absorption. Handling chemicals in the fume hood reduces the inhalation hazard and provides protection from splashes. Use of proper gloves and other PPE will prevent skin exposure and damage.
Gloves are used to protect hands and in some cases portions of the arms from coming into contact with a hazard. In the laboratory the main hazards involve chemicals, biological material, radioactive materials, sharp objects, and extreme temperatures (autoclave, liquid nitrogen).
Glove selection is based on the following factors to determine the best option for each task.
Contact EH&S if you have any concerns or questions or would like guidance with glove selection.
Disposable gloves are thin (< 8 mil) and most commonly made from latex rubber, nitrile rubber, polyvinyl chloride, or Neoprene. Disposable latex gloves typically offer sufficient protection when handling small quantities or diluted chemicals with a low chance for contact or splash. They are not designed for applications involving prolonged, direct exposure to chemicals, but instead for incidental splash exposures. Nitrile gloves provide protection from a wider range of hazardous materials and are more resistant to tearing. Table 6.1.2.1 shows glove recommendation for handling small quantities of common lab chemicals.
Disposable gloves must not be reused.
If chemical contamination occurs while wearing disposable gloves, immediately remove and discard the gloves. If contamination results from incidental contact (small amounts of chemicals that will dry quickly), remove the gloves and dispose of them in the regular garbage. If gloves are grossly contaminated (were immersed in, saturated with, or are still wet with chemicals), they should be collected as hazardous waste in a plastic bag as described in Chapter VI, Section 4.2.4, Solid Chemical Wastes. After removing contaminated gloves, wash your hands and don a new pair of gloves. Contact EH&S for assistance in determining the best disposal option for gloves contaminated by a particular chemical.
Table 6.1.2.1: Glove Recommendations for Small Quantity Handling |
||
Hazardous Material |
Latex Exam Gloves* |
Best N-Dex 7005 (4 mil) |
---|---|---|
Acetic acid |
NT |
x |
Acetone |
NT |
x |
Acetonitrile |
NT |
x |
Acrylamide |
NT |
x |
Chloroform |
NT |
x |
DMSO |
NT |
x |
Ethanol |
NT |
x |
Ethidium bromide |
NT |
x |
Methanol |
NT |
x |
Phenol |
NT |
x |
Sodium hypochlorite |
NT |
x |
Blood and body fluids |
x |
|
Radioactive isotopes |
x |
|
NT = Not Tested x = Best Glove Choice |
Reusable gloves are thicker and offered in a wider range of materials. The most common are latex rubber, nitrile rubber, Neoprene, Viton, or butyl rubber. These gloves are necessary when handling large quantities or chemicals where significant contact or splash is likely. Once contaminated: they may be washed and dried. Signs of degradation include tears, holes, cracking, swelling, hardening, etc., and if found, the gloves must be properly disposed as outlined in 6.1.2. Table 6.1.2.2 shows glove recommendation for handling large quantities of common lab chemicals.
Table 6.1.2.2: Specialty Gloves for Large Quantity Handling |
||||||
Hazardous Material |
Ansell Edmont Natural Rubber |
Ansell Edmont Neoprene (15 mil) |
Best Nitro-Solv 727 (15 mil) |
North Butyl Rubber (17 mil) |
Ansell Edmont Supported PVA |
Ansell Edmont 4H |
---|---|---|---|---|---|---|
Acetic acid |
X |
X |
|
|
|
X |
Acetone |
X |
X |
|
X |
|
|
Acetonitrile |
X |
X |
|
X |
|
X |
Acrylamide |
|
X |
X |
|
|
|
Chloroform |
|
|
|
|
X |
X |
DMSO |
X |
X |
X |
X |
|
|
Ethanol |
X |
X |
|
X |
|
X |
Ethidium bromide |
X |
X |
|
|
|
|
Methanol |
X |
X |
|
|
|
|
Phenol |
X |
X |
|
X |
X |
|
Sodium hypochlorite |
X |
X |
X |
|
|
|
X = Best Glove Choice |
Gloves should not be worn in elevators, restrooms, library, conference rooms, cafeterias (or other eating areas), stairs, offices, or non-lab floors.
If you are wearing gloves, remove them before answering the phone or touching equipment or doorknobs. This will prevent contamination of facilities and other personnel if your gloves are contaminated. Conversely, it will prevent contamination of your gloves if they are being used to protect the material you are handling.
Use a secondary container to transport materials. It will allow you to remove your gloves as well as protect your work.
A latex allergy is an allergy to products made from natural rubber latex. It is usually a reaction to proteins in the rubber that are still present in products made from natural rubber latex. In the case of powdered latex gloves, it has been determined that the corn starch powder is the carrier of the latex allergen. A recent study demonstrated that corn starch binds with the allergenic latex proteins and transports them by direct contact with the skin and/or by exposure to airborne particles.
Latex allergies can produce a variety of symptoms. The severity of the reaction depends on the potency of the allergen, its concentration, the duration of exposure, and the individual's sensitivity. Factors that exacerbate the severity of the contact dermatitis include friction, pressure, sweating, and previous dermatological disease. Currently, the rate of latex allergy prevalence among lab and healthcare workers has been estimated between 6-14%, compared with 1% in the general population.
There is no treatment available for latex allergies. The only means of controlling allergic reactions is strict avoidance of latex exposure. Employees who have been diagnosed with latex allergies should:
Fred Hutch prohibits the use of powdered latex gloves, but does permit the use of nonpowdered latex gloves. This is to prevent employees from developing latex allergies and to protect employees who have latex allergies. The powder carries the latex and, when airborne, it can trigger a response in those who are sensitive. The airborne powder contributes to the development of allergies to latex. If you prefer a powdered glove, you can use a powdered nitrile glove such as Microflex, Nitron One, or N-dex. In general, a nitrile glove will provide better protection when handling chemicals and is recommended.
Double gloving is recommended under several circumstances to provide adequate protection. It is important to double glove when handling millicurie amounts of 125I or 131I, changing the outer glove frequently. Double gloving is required when handling HIV cultures or when human blood routinely contacts gloved hands. If you are handling a highly toxic or a dangerously toxic chemical, but still need good dexterity to perform the work, wearing latex over nitrile gloves will provide adequate protection without impeding dexterity.
Glove recommendations in the charts and databases are typically based on permeation and degradation testing. In general, manufacturers use the following for assessing glove chemical resistance.
Note: It is very important to understand each manufacturer's definitions and source of data. Some manufacturers rely on reference materials while others use data from one glove for all gloves of similar material and thickness. Others only publish data for testing done on each glove material and thickness and communicate if a glove has not been tested against materials in the table. Below is a list of some common vendors with links to their glove interactive database or charts.
Manufacture |
Interactive Database |
Chart |
---|---|---|
Ansell |
AnsellPro SpecWare (for thin lab gloves, select Splash Guide results) |
For thicker gloves only: Ansell_8th Edition Chemical Resistance Guide |
Best |
Showa (select US then Chemrest – Chemical Resistance Guide) |
N/A |
Kimberly-Clark |
N/A |
|
Microflex |
N/A |
Microflex Product Literature (for Microflex thin latex and nitrile gloves), select Microflex Chemical Resistance Guide) |
Lab coats are available for all employees whose jobs involve direct use of hazardous materials (radioactive, chemical, blood, body fluid, and infectious materials). The lab coat service was approved by the Health & Safety Committee and the Space and Resources Committee and there are several locations throughout the Hutch where lab coats are stored. A vendor provides weekly laundry service for all the lab coats.
Generic lab coats with Fred Hutch logos are available for laboratory employees who handle hazardous materials in the Weintraub, Hutchinson, Thomas, and PHS buildings. Personalized lab coats in these locations are not provided by EH&S. The new generic coats will be available in a range of sizes in each location. Please take only one coat as needed, and return it to the hamper when it becomes soiled. See the EH&S Lab Coat Service for more information.
New lab coats are picked up and soiled coats returned to the following locations:
Distribution Location |
|
Fairview |
LF-260-3; 2nd floor, west corner |
Hutchinson |
C1M-029: First level interstitial, Building C, northwest corner, past the vending machines |
PHS |
E-Level Clinic: ME-B502 Fifth floor labs: Hallway next to M5-A402 |
Thomas |
Alcove: Cross-hallway of E-level, near the west set of elevators |
Weintraub |
AD-115: Near the D-level elevators |
Only PHS clinic lab coat users may order new lab coats from EH&S. Fill out the Lab Coat Order Form or call in an order directly to EH&S. Please include the following with your request:
Measure the widest portion of your chest and choose a lab coat 1 to 2 sizes larger, for wearing ease. The lab coats range from 34 to 54 (even sizes).
Please allow up to three weeks for delivery of your new lab coat. Contact EH&S if a loaner coat is necessary.
As a minimum standard, safety glasses must be worn whenever handling hazardous materials.
Approved safety glasses are those which meet the American National Standards Institute (ANSI) Z87 testing criteria. Approved glasses are marked with the number Z87 on the inside of the temple. Do not remove side shields from these glasses. Safety glasses offer good impact protection, but very limited splash protection.
The eyes and face should be protected from potential splashes by conducting work with hazardous substances in a chemical fume hood.
Chemical splash goggles underneath a face shield must be worn when a chemical fume hood is not used and there is a potential for a hazardous substance to splash.
If you choose to wear contacts when handling chemicals, be sure to wear safety glasses. In the event of a chemical splash to the eyes, contacts can hold the chemical against the eye prolonging the exposure and increasing eye damage.
Lab ventilation and chemical fume hoods typically control exposure to hazardous chemicals. EH&S will medically clear, fit test, train, and issue a cartridge respirator to an employee if there is a potential for overexposure.
Do not purchase respiratory protective devices of any kind without EH&S approval. If you believe a hazardous airborne exposure condition may exist in your work area, contact EH&S.
Employees working in a lab must wear sturdy-soled, well-fitting shoes that cover the entire foot. Sandals, slip-ons, perforated shoes (e.g., Crocs™), and open-toe shoes are not acceptable in labs. Leather shoes are recommended.